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Also, these are mostly used with internal supplementation for better overall results

platensis strain LB 2340 from the Texas Algal Culture collection UTEX was grown under constant white light using an alkaline Spirulina medium: solution I (162 mM NaHCO 3 , 38 mM Na 2 CO 3 , and 2.9 mM K 2 HPO 4 in 500 ml dH 2 O) and II (29.4 mM NaNO 3 , 5.74 mM K 2 SO 4 , 17.1 mM NaCl, 0.81 mM MgSO 4 , 0.27 mM CaCl 2 in 500 ml dH 2 O) were autoclaved separately, combined after cooling, and 2 ml of a sterile-filtered 0.1 mM vitamin B 12 solution was added

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